Yeast Structural Genomics
CNRS UMR8619
Université Paris Sud - Orsay
IFR 115 Génome
Génopole Evry

Persons in charge

General Presentation

The IBBMC is housing the Yeast Structural Genomics. Our initial pilot project concerned the structure determination of yeast proteins of unpredicted fold. Within this program we have set up a technological platform for the high throughput determination of 3 dimensional structures protein. This setup includes:

  cloning and expression of proteins
  purification and characterization of proteins
  protein crystallization
  3D structure determination by X-ray diffraction analysis
  bioinformatics analysis and data management

In order to accelerate the pace, we have introduced an automatic approach for many of the steps of the complex road leading from gene to 3D structure. The ensemble of technologies is organized now in a platform that is open to the scientific community and that has acquired the label RIO. The following services are offered:

  semiautomatic cloning and expression testing in bacterial hosts
  purification and, if necessary refolding, of overexpressed proteins
  crystallization screening of purified proteins or protein complexes
  3D structure determination and structural analysis
  bioinformatic analysis of the protein sequences
  software for experimental data storage and browsing

Services

  Cloning
  Expression tests
  Large scale expression
  Purification
  Crystallization
  Structure determination
  Computing services